Expression of miR-140-3p in synovial fluid of knee osteoarthritis patients reflects the progression of osteoarthritis

doi:10.3969/j.issn.2095-4344.2016.35.016

Abstract

Abstract:

BACKGROUND: Osteoarthritis is an age-related joint disease characterized by articular cartilage degeneration.

OBJECTIVE: To assess the expression of miR-140-3p in synovial fluid of knee osteoarthritis, and identify whether miR-140-3p levels in synovial fluid are correlated with disease severity.

METHODS: We collected 10 healthy volunteers, 10 patients with gouty arthritis, 10 patients with rheumatoid arthritis, and 45 patients with knee osteoarthritis (15 cases in early stage, 15 cases in middle stage and 15 cases in the late stage). Real-time fluorescence quantitative PCR was used to detect miR-140-3p expression in synovial fluid in each group.

RESULTS AND CONCLUSION: (1) Expression of miR-140-3p was significantly down-regulated in knee osteoarthritis group. Significant differences in miR-140-3p expression were detected between non-knee osteoarthritis group and knee osteoarthritis group (P < 0.05). No significant difference in miR-140-3p expression was determined among healthy control group, gouty arthritis group and rheumatoid arthritis group (P > 0.05). (2) The miR-140-3p expression in the healthy control group was 11.4 times that in the knee osteoarthritis group. The expression of miR-140-3p decreased with the increase of the severity of osteoarthritis. (3) Spearman rank correlation analysis suggested that the expression of miR-140-3p was negatively correlated with osteoarthritis severity. (4) Results suggest that the expression of miR-140-3p in synovial fluid can reflect the progression of knee osteoarthritis to a certain extent.

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

Key words: Osteoarthritis, Knee, Synovial Fluid, MicroRNAs, Polymerase Chain Reaction, Tissue Engineering

CLC Number:

R318

Yin Chun-ming, Pan Xiao-hua. Expression of miR-140-3p in synovial fluid of knee osteoarthritis patients reflects the progression of osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(35): 5277-5283.

Figures/Tables 7

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